Lab reagents, strains and protocols


Please address requests to h.roehl(at)sheffield.ac.uk


Reagents:


RNA constructs:


PCSII lynCherry (map) (genbank)

PCSII lynTomato (map) (genbank)

PCSII lynGFP (map) (genbank)

PCSII lynCerulean (map) (genbank)


emission spectra.pdf

excitation spectra.pdf



Probe template constructs:


colIa2

colXa1

osterix

runx2a

runx2b

pea3

erm2

tcf7

osn

cvl

sox9a

gdf5

slc35b2

sparc

ext2

gfra3

myf5

myod1

tbx1

tcf21

ret1

ptc1

ptc2

twist2

cebpa

Fish  Strains:


dlx5:gfp

dak(to273b)

dak(tw25e)

dak(tf205)

bmp5

ret1(ig391)

ret1(hu2846)

uas:dnret

hs-noggin3

hs-fgf3 cherry

hs-dnfgfr1

koi(tl226d)

pea3(hu3536)

pic(to14mx)

ppt(ti265)

gfra3(hh024)

bact:H2B Cherry

bact:GFP

wnt5a(sa93)

hs-dkk1

hs-wnt7

cpt(m169)

Protocols:


Double labelling of cartilage and muscle:

Roehl:Col2-muscle.pdf







Double labelling of cartilage and bone:

AlcianvonKossa.pdf







Lab in situ protocol

Roehl Lab in situ.pdf

 


 

Roehl Lab